Mouse NF-L Assay for Preclinical Biomarker Research
Neurofilament light chain (NF-L) is a biomarker of axonal injury and neuronal damage used across neurodegeneration and neurological disease research. In mouse models, NF-L can provide a valuable translational readout for studying disease progression, neuronal injury, and therapeutic response.
However, mouse biomarker studies are often limited by sample volume.
Assays that require higher volumes often demand terminal sampling or even combining multiple animals for a single data point, increasing animal use and making longitudinal studies impossible.
SPEAR UltraDetect™ Mouse NF-L helps address these challenges by enabling ultra-sensitive detection of NF-L in minimal volumes, supporting more flexible preclinical study designs and longitudinal NF-L monitoring from the same animal over time.
Ultra-Sensitive, Low-Volume Mouse NF-L Measurement Across Plasma, Serum, and CSF
SPEAR UltraDetect™ Mouse NF-L supports sensitive neurofilament light chain measurement across key mouse sample types, including plasma, serum, and CSF.
In commercially sourced samples from young mice, NF-L was measured across plasma, serum*, and CSF* using 1 µL diluted sample. This flexibility supports preclinical studies where sample volume is limited and researchers need to evaluate NF-L across multiple matrices.
By combining ultra-sensitivity with low sample input, SPEAR UltraDetect™ Mouse NF-L helps researchers preserve valuable mouse samples, support longitudinal study designs, and measure NF-L across multiple sample types used in preclinical neuroscience research.
SPEAR UltraDetect™ Mouse NF-L
[1 µL diluted sample]
*Endogenous levels for serum and CSF were tested for reference only. Full verification of assay performance is recommended before using these matrices in studies.
Assay Specifications
| MRD | 8X |
| Diluted Sample Volume | 1 µL |
| LOD (pg/mL) | 0.037 |
| fLLOQ (pg/mL) | 1.00 |
| Spike-recovery (% recovery) | 93.4% |
| Linearity (% recovery) | 101% |
| Intra-plate CV (AVG%) | 1.1% |
| Inter-plate CV (AVG%) | 8.8% |
| EDTA Plasma Quantifiability | 100% |
Designed to Expand What Is Possible in Mouse NF-L Studies
Preserve limited mouse samples and support repeated sampling across multiple study timepoints.
Monitor NF-L changes from the same animal over time to support disease progression or therapeutic response studies.
Generate more biomarker data from each animal and support study designs that may reduce reliance on larger terminal cohorts.
Some workflows adapt human NF-L assays for mouse sample testing. SPEAR UltraDetect™ Mouse NF-L is designed for mouse sample analysis and uses a mouse-optimized assay diluent.
This supports improved matrix compatibility, parallelism, and dilution linearity in mouse samples, helping researchers generate reliable NF-L measurements in low-volume preclinical studies.
