2-Factor Authentication: the Foundation of SPEAR Biomarker Technology

Biomarker technology, in terms of protein quantification, has advanced beyond traditional immunoassays, which lack intrinsic signal amplification. Early immuno-PCR methods faced high non-specific background due to transient DNA oligo interactions. Innovations like proximity extension and digital ELISA improved sensitivity, but require high-affinity antibodies, complex workflows and/or proprietary instrumentation, while still being prone to transient interactions.

Successive Proximity Extension Amplification Reaction (SPEAR) overcomes these limitations with an ultra-sensitive and ultra-specific assay. Utilizing ubiquitous qPCR for amplification and a patented two-factor authentication mechanism, SPEAR achieves superior assay performance with a homogeneous, wash-free format and simplified workflow. This breakthrough in biomarker technology enhances protein measurement, offering both the sensitivity and specificity required for advanced research applications such as the detection of low-abundant, blood-based proteins.

Download our tech note for a detailed description of the mechanism behind our patented, two-factor target authentication, the theory and proof behind SPEAR ultra-sensitive biomarker detection, and the unique advantages of adopting SPEAR Technology for your research.